Sputum test
Sputum test

Sputum smear (KOH test)

Definition:
This is a test to detect the presence of fungal microorganisms in sputum.

Alternative Names:
KOH test; Fungal smear

How the test is performed:
Obtain a sputum sample by coughing deeply and expelling the material that comes from the lungs (sputum) into a specimen container. A smear of sputum is prepared with a potassium hydroxide (KOH) and examined under the microscope. If it is positive, fungal cells can be observed.
How to prepare for the test:
There is no special preparation.
How the test will feel:
There is no discomfort.
Why the test is performed:
The test may be performed when there is abnormal sputum production or when an infection of the lungs is suspected.
Normal Values:
No presence of organisms is normal.
What abnormal results mean:
Abnormal results indicate the presence of fungus such as histoplasmosis, coccidioidomycosis, or blastomycoses. White blood cells called eosinophils may also be present.

Additional conditions under which the test may be performed:
What the risks are:
There are no risks.
Special considerations:
Not applicable.

Review Date: 1/13/2002
Reviewed By: Camille Kotton, M.D., Infectious Diseases Division, Massachusetts General Hospital and Brigham and Women's Hospital, Boston, MA. Review provided by VeriMed Healthcare Network.
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