Viral lesion culture
Viral lesion culture

Skin lesion KOH exam

Definition:
The skin lesion KOH exam is a test to diagnose a fungal infection of the skin.

Alternative Names:
Potassium hydroxide examination of skin lesion; Fungal wet prep

How the test is performed:
The lesion is scraped, using a blunt edge such as the edge of a microscope slide. The scrapings from the skin lesion are placed in a solution of potassium hydroxide (KOH) and examined under the microscope. The fungus can be seen, if present. This is different from a saline (salt water) examination, since the KOH destroys all non-fungal cells, making it much easier to see the fungal material.
How to prepare for the test:
Adults:
There is no special preparation for the test.

Infants and children:
The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experiences, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:
How the test will feel:
You may feel a pressure sensation when the lesion is scraped from the skin.
Why the test is performed:
The test is performed when fungal infections of the skin are suspected.
Normal Values:
The lack of fungus is normal and expected.
What abnormal results mean:
The KOH smear shows hyphae or fungal organisms. This may indicate ringworm (tinea corporis), athlete's foot, jock itch, or many other fungal infections.
What the risks are:
There is a small risk of bleeding or infection from scraping the lesion.
Special considerations:
Not applicable.

Review Date: 1/8/2002
Reviewed By: Camille Kotton, M.D., Infectious Diseases Division, Massachusetts General Hospital and Brigham and Women's Hospital, Boston, MA. Review provided by VeriMed Healthcare Network.
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